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Hesperetin was the only ligand to interact with amino acid GLU Furthermore, hesperetin and malvidin O -glucoside were the only two ligands to interact with the amino acid LEU5. The anthocyanidin malvidin had the lowest binding affinity for this domain. The conformations can be compared visually in Fig 5D. Given the similarity between the known and experimental conformations of myristic acid, the computational methodology used showed to produce conformations similar to those found in known structures, and was therefore used in this study.

Hydrogen bonds are shown as one headed, dashed arrows formed with amino acid main-chain green or side-chain blue atoms, while pink double headed, dashed arrows represent charged interactions. Bands within the same membrane that were not quantified were cropped out for clarity See supplementary S1 Fig for full membrane images. The best conformation of delphinidin O -arabinoside binds to the opposite face of the transcription domain than where the domain binds to DNA, indicating that delphinidin O -arabinoside is predicted to interact with this domain even if the domain is interacting with DNA.

The experimental and crystallographic conformations of myristic acid had a root-mean square deviation RMSD value less than two angstroms 1. An RMSD value of less than two angstrom between a known conformation and a conformation acquired through an experimental docking scheme is commonly used in computational molecular docking to determine if a docking scheme can give valid predictions [ 21 ].

The differences in the experimental and crystallographic conformations of myristic acid are probably due to fact that the myristic acid ligand used to validate the computational scheme was allowed to rotate as shown by the rotated experimental conformation in Fig 5B. Rotation of ligand bonds allows for a more realistic simulation as compounds are rarely rigid structures in nature. This would allow for the observed interactions of this study to be connected to possible pre-transcriptional regulation of proteins associated with type II diabetes. The lanes in each membrane are labeled by the anthocyanin treatment the cells received.

Browse Subject Areas? Click through the PLOS taxonomy to find articles in your field. This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: The authors received no specific funding for this work. Materials and Methods Molecular Docking Ligand preparation. Download: PPT. Fig 1. Molecular structures of berry and citrus compounds studied. Molecular docking. Validation of molecular docking methodology. Preparation of Anthocyanin Extracts from Blueberry and Blackberry Fermented Beverages Anthocyanin ANC extracts from fermented blueberry and blackberry beverages were prepared as described previously [ 19 ].

Table 1. Fig 2. Table 2. Fig 3. Table 3. Fig 4. Fig 5. Fig 6. Fig 7. Supporting Information. S1 Fig. S2 Fig. References 1. Mol Cell. Nat Struct Biol. View Article Google Scholar 4. Mol Med. Biochem Biophys Res Commun. Biochim Biophys Acta. View Article Google Scholar 9. Selecting the most appropriate board website is […]. A virtual data center is a pool area of cloud infrastructure assets that are designed to be scalable and flexible for enterprise workloads. Cost Savings and Efficiency: Using a virtual info center, establishments can reduce the hardware […]. The best mac pc antivirus is known as a combination of safeguards, features, and affordability.

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